PrimePCR™ SYBR® Green Assay: GOLGA6B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040128

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GOLGA6B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GOLGA6B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037405
Assay Design:   exonic
Chromosome Location:   15:72954618-72954762question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000435680 ENST00000425768 ENST00000450633 ENST00000300576 ENST00000434739 ENST00000378060 ENST00000382483 ENST00000449352 ENST00000265978 ENST00000524416 ENST00000254958 ENST00000423891 ENST00000421285 ENST00000533783 ENST00000265968 ENST00000290438 ENST00000378386 ENST00000274747 ENST00000305544 ENST00000418109

Gene Information

This gene is found in a large low copy repeat sequence or duplicon that is found in multiple copies which are greater than 90% similar on chromosome 15. Duplicons are associated with deletions inversions and other chromosomal rearrangements that underlie genomic disease. This gene is a member of the golgin gene family whose protein products localize to the Golgi apparatus. The majority of the related gene copies are thought to be transcribed pseudogenes. It is not known whether this gene is a pseudogene or if it encodes a golgin protein. [provided by RefSeq Jul 2008]

Gene Symbol:   GOLGA6B
Gene Name:   golgin A6 family, member B
Aliases:   GOLGA, GOLGA6D
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000215186
Entrez:   55889
Chromosome Mapping:   15q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.970000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download