PrimePCR™ SYBR® Green Assay: GTF2H4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040120

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037397
Assay Design:   exonic
Chromosome Location:   6:30877738-30878466question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000395290 ENST00000531165 ENST00000524993 ENST00000430170 ENST00000445164 ENST00000526090 ENST00000394894 ENST00000508792 ENST00000569065 ENST00000527954 ENST00000577226 ENST00000264659 ENST00000578925 ENST00000542707 ENST00000290607 ENST00000456968 ENST00000435498 ENST00000416161 ENST00000356861 ENST00000450764 ENST00000592287 ENST00000588216 ENST00000425528 ENST00000441499 ENST00000434226 ENST00000416773 ENST00000454718 ENST00000320389 ENST00000404283 ENST00000498729 ENST00000440824 ENST

Gene Information

Description Not Available

Gene Symbol:   GTF2H4
Gene Name:   general transcription factor IIH, polypeptide 4, 52kDa
Aliases:   TFB2, TFIIH
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000213780
Entrez:   2968
UniGene:   Hs.485070
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.640000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download