PrimePCR™ SYBR® Green Assay: GPSM3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040116

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GPSM3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GPSM3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037393
Assay Design:   exonic
Chromosome Location:   6:32158791-32158936question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000472817 ENST00000424520 ENST00000457070 ENST00000529721 ENST00000336898 ENST00000532435 ENST00000443276 ENST00000404583 ENST00000314567 ENST00000404744 ENST00000407775 ENST00000520492 ENST00000429209 ENST00000441705 ENST00000301067 ENST00000472154 ENST00000453667 ENST00000420041 ENST00000494847 ENST00000383265 ENST00000383269 ENST00000353265 ENST00000339818 ENST00000479498 ENST00000414839 ENST00000448684 ENST00000474364 ENST00000432871 ENST00000445326 ENST00000487761 ENST00000375040 ENST

Gene Information

Description Not Available

Gene Symbol:   GPSM3
Gene Name:   G-protein signaling modulator 3
Aliases:   AGS4, C6orf9, G18, G18.1a, G18.1b, G18.2, NG1
RefSeq:   NC_000006.11 NG_028190.1 NT_007592.15 NT_113891.2 NT_167244.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000213654
Entrez:   63940
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.060000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download