PrimePCR™ SYBR® Green Assay: RP11-632K20.1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040108

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037385
Assay Design:   exonic
Chromosome Location:   15:32744694-32744974question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000532622 ENST00000526619 ENST00000337838 ENST00000450328 ENST00000525590 ENST00000329523 ENST00000563027 ENST00000340249 ENST00000567927 ENST00000341650 ENST00000569052 ENST00000327271 ENST00000544495 ENST00000562783 ENST00000333156 ENST00000566740 ENST00000512626 ENST00000509311 ENST00000562377 ENST00000414865 ENST00000569659 ENST00000448387 ENST00000426622 ENST00000359187 ENST00000360553 ENST00000432566 ENST00000543376 ENST00000342314 ENST00000267731 ENST00000438958 ENST00000450802 ENST

Gene Information

Description Not Available

Gene Symbol:   RP11-632K20.1
Gene Name:   Putative golgin subfamily A member 6-like protein 11
Aliases:   Not Available
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000206127
Entrez:   728047
UniGene:   Hs.568189
Chromosome Mapping:   15q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.030000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download