PrimePCR™ SYBR® Green Assay: PPP1R11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040078

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PPP1R11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PPP1R11, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037355
Assay Design:   exonic
Chromosome Location:   6:30037736-30037834question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000433942 ENST00000452679 ENST00000417223 ENST00000436833 ENST00000428563 ENST00000449435 ENST00000441553 ENST00000437937 ENST00000446310 ENST00000414618 ENST00000434591 ENST00000452227 ENST00000455350 ENST00000448378 ENST00000429382 ENST00000418211 ENST00000444101 ENST00000430959 ENST00000376773 ENST00000376772 ENST00000376769 ENST00000376765 ENST00000376763 ENST00000376758 ENST00000430828 ENST00000436591 ENST00000448210 ENST00000444994 ENST00000412077 ENST00000422273 ENST00000400658 ENST

Gene Information

This gene encodes a specific inhibitor of protein phosphatase-1 (PP1) with a differential sensitivity toward the metal-independent and metal-dependent forms of PP1. The gene is located within the major histocompatibility complex class I region on chromosome 6. [provided by RefSeq Jul 2008]

Gene Symbol:   PPP1R11
Gene Name:   protein phosphatase 1, regulatory (inhibitor) subunit 11
Aliases:   HCG-V, HCGV, IPP3, MGC125741, MGC125742, MGC125743, TCTE5, TCTEX5
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204619
Entrez:   6992
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.970000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download