PrimePCR™ SYBR® Green Assay: GNL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040071

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037348
Assay Design:   exonic
Chromosome Location:   6:30522832-30523335question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000417834 ENST00000430236 ENST00000310799 ENST00000381569 ENST00000265801 ENST00000437917 ENST00000443235 ENST00000299721 ENST00000383596 ENST00000458592 ENST00000428189 ENST00000435788 ENST00000376621 ENST00000433809 ENST00000441604 ENST00000450423 ENST00000454829 ENST00000416639 ENST00000377647 ENST00000456183 ENST00000356699 ENST00000377509 ENST00000429767 ENST00000377621 ENST00000355832 ENST00000542458 ENST00000332857 ENST00000355045 ENST00000337090 ENST00000347528 ENST00000289734 ENST

Gene Information

The GNL1 gene identified in the human major histocompatibility complex class I region shows a high degree of similarity with its mouse counterpart. The GNL1 gene is located less than 2 kb centromeric to HLA-E in the same transcriptional orientation. GNL1 is telomeric to HLA-B and HLA-C. [provided by RefSeq Jul 2008]

Gene Symbol:   GNL1
Gene Name:   guanine nucleotide binding protein-like 1
Aliases:   DKFZp547E038, DKFZp547E128, FLJ37752, HSR1
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204590
Entrez:   2794
UniGene:   Hs.83147
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.270000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download