PrimePCR™ SYBR® Green Assay: CCHCR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040064

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037341
Assay Design:   exonic
Chromosome Location:   6:31124532-31124621question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000416392 ENST00000447252 ENST00000452333 ENST00000456365 ENST00000447874 ENST00000453360 ENST00000457929 ENST00000432736 ENST00000416850 ENST00000433804 ENST00000419528 ENST00000428677 ENST00000439536 ENST00000438739 ENST00000425351 ENST00000423825 ENST00000427654 ENST00000438424 ENST00000383341 ENST00000400352 ENST00000414614 ENST00000449364 ENST00000438812 ENST00000437918 ENST00000417501 ENST00000444855 ENST00000416552 ENST00000416163 ENST00000400351 ENST00000425620 ENST00000444737 ENST

Gene Information

Description Not Available

Gene Symbol:   CCHCR1
Gene Name:   coiled-coil alpha-helical rod protein 1
Aliases:   C6orf18, HCR, MGC126371, MGC126372, SBP
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000204536
Entrez:   54535
UniGene:   Hs.485075
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.550000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download