PrimePCR™ SYBR® Green Assay: PRAMEF13, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040054

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PRAMEF13, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PRAMEF13, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037331
Assay Design:   exonic
Chromosome Location:   1:13448104-13448233question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000434886 ENST00000416192 ENST00000399074 ENST00000422950 ENST00000396560 ENST00000396559 ENST00000356427 ENST00000324570 ENST00000438480 ENST00000424530 ENST00000399088 ENST00000399065 ENST00000413089 ENST00000360428 ENST00000434452 ENST00000256646 ENST00000332296 ENST00000400814 ENST00000240189 ENST00000376132 ENST00000334600 ENST00000344998 ENST00000276201 ENST00000345865 ENST00000321945 ENST00000519811 ENST00000518668 ENST00000262585 ENST00000424248 ENST00000581899

Gene Information

Description Not Available

Gene Symbol:   PRAMEF13
Gene Name:   PRAME family member 13
Aliases:   Not Available
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000204495
Entrez:   400736
Chromosome Mapping:   1p36.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.690000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download