PrimePCR™ SYBR® Green Assay: PRAMEF20, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040049

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PRAMEF20, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PRAMEF20, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037326
Assay Design:   exonic
Chromosome Location:   1:13747431-13747568question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000347901 ENST00000404826 ENST00000389531 ENST00000357726 ENST00000437584 ENST00000535591 ENST00000235349 ENST00000235347 ENST00000330881 ENST00000361079 ENST00000355096 ENST00000376192 ENST00000376189 ENST00000415464 ENST00000376182 ENST00000414205 ENST00000423177 ENST00000376168 ENST00000357367 ENST00000376152 ENST00000327795 ENST00000332192 ENST00000330087 ENST00000602960 ENST00000316412 ENST00000568276

Gene Information

Description Not Available

Gene Symbol:   PRAMEF20
Gene Name:   PRAME family member 20
Aliases:   Not Available
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000204478
Entrez:   645425
Chromosome Mapping:   1p36.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.480000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download