PrimePCR™ SYBR® Green Assay: AIF1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040047

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037324
Assay Design:   exonic
Chromosome Location:   6:31584642-31584736question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000396950 ENST00000440907 ENST00000412407 ENST00000424944 ENST00000549432 ENST00000355832 ENST00000376059 ENST00000337917 ENST00000376049 ENST00000413349 ENST00000437985 ENST00000415830 ENST00000425748 ENST00000454372 ENST00000416422 ENST00000414149 ENST00000454573 ENST00000419376 ENST00000383474 ENST00000417200 ENST00000383473

Gene Information

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2008]

Gene Symbol:   AIF1
Gene Name:   allograft inflammatory factor 1
Aliases:   AIF-1, IBA1, IRT-1, IRT1
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204472
Entrez:   199
UniGene:   Hs.76364
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.320000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download