PrimePCR™ SYBR® Green Assay: LY6G6F, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040041

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037318
Assay Design:   exonic
Chromosome Location:   6:31675280-31675454question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000325811 ENST00000349780 ENST00000416449 ENST00000425647 ENST00000360822 ENST00000359309 ENST00000442105 ENST00000556331 ENST00000414847 ENST00000446062 ENST00000557698 ENST00000439683 ENST00000390654 ENST00000375832 ENST00000556581 ENST00000503322 ENST00000455955 ENST00000453044 ENST00000455632 ENST00000554860 ENST00000428105 ENST00000430366 ENST00000447811 ENST00000400084 ENST00000383420 ENST00000395192 ENST00000398906

Gene Information

The human G6f protein is a type I transmembrane protein belonging to the immunoglobin (Ig) superfamily which is comprised of cell-surface proteins involved in the immune system and cellular recognition (de Vet et al. 2003 [PubMed 12852788]).[supplied by OMIM Mar 2008]

Gene Symbol:   LY6G6F
Gene Name:   lymphocyte antigen 6 complex, locus G6F
Aliases:   C6orf21, G6f, LY6G6D, NG32
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204424
Entrez:   259215
UniGene:   Hs.591794
Chromosome Mapping:   6p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.720000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download