PrimePCR™ SYBR® Green Assay: DOM3Z, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040028

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DOM3Z, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DOM3Z, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037305
Assay Design:   exonic
Chromosome Location:   6:31938383-31938524question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000400314 ENST00000400310 ENST00000400309 ENST00000400308 ENST00000400305 ENST00000449640 ENST00000427690 ENST00000426498 ENST00000453742 ENST00000399936 ENST00000399937 ENST00000383330 ENST00000345382 ENST00000569704 ENST00000337523 ENST00000375349 ENST00000495340 ENST00000375356 ENST00000419797 ENST00000446967 ENST00000427627 ENST00000443250 ENST00000439989 ENST00000454430 ENST00000458308 ENST00000432110 ENST00000414092

Gene Information

This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. The function of its protein product is unknown but its ubiquitous expression and conservation in both simple and complex eukaryotes suggests that this may be a housekeeping gene. [provided by RefSeq Jul 2008]

Gene Symbol:   DOM3Z
Gene Name:   dom-3 homolog Z (C. elegans)
Aliases:   DOM3L, NG6, RAI1
RefSeq:   NC_000006.11 NG_000013.3 NG_004658.2 NG_005163.2 NT_007592.15 NT_113891.2 NT_167245.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204348
Entrez:   1797
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.390000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download