PrimePCR™ SYBR® Green Assay: COL11A2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040013

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037290
Assay Design:   exonic
Chromosome Location:   6:33154423-33154556question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000549491 ENST00000547561 ENST00000552971 ENST00000550101 ENST00000548739 ENST00000553240 ENST00000549836 ENST00000383088 ENST00000420405 ENST00000383219 ENST00000443138 ENST00000447855 ENST00000434780 ENST00000548637 ENST00000548137 ENST00000374708 ENST00000457788 ENST00000395194 ENST00000341947 ENST00000357486 ENST00000374714 ENST00000374713 ENST00000395197 ENST00000374712 ENST00000361917 ENST00000426824 ENST00000397488 ENST00000562208 ENST00000568887 ENST00000324385 ENST00000421665 ENST

Gene Information

This gene encodes one of the two alpha chains of type XI collagen a minor fibrillar collagen. It is located on chromosome 6 very close to but separate from the gene for retinoid X receptor beta. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Proteolytic processing of this type XI chain produces PARP a proline/arginine-rich protein that is an amino terminal domain. Mutations in this gene are associated with type III Stickler syndrome otospondylomegaepiphyseal dysplasia (OSMED syndrome) Weissenbacher-Zweymuller syndrome autosomal dominant non-syndromic sensorineural type 13 deafness (DFNA13) and autosomal recessive non-syndromic sensorineural type 53 deafness (DFNB53). Alternative splicing results in multiple transcript variants. A related pseudogene is located nearby on chromosome 6. [provided by RefSeq Jul 2009]

Gene Symbol:   COL11A2
Gene Name:   collagen, type XI, alpha 2
Aliases:   DFNA13, DFNB53, HKE5, PARP, STL3
RefSeq:   NC_000006.11 NG_023374.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1 NG_011589.1
Ensembl:   ENSG00000204248
Entrez:   1302
UniGene:   Hs.390171
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.380000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download