PrimePCR™ SYBR® Green Assay: RING1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040010

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RING1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RING1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037287
Assay Design:   exonic
Chromosome Location:   6:33177450-33177560question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000310109 ENST00000527115 ENST00000415941 ENST00000383212 ENST00000375663 ENST00000444930 ENST00000435657 ENST00000374656 ENST00000425144 ENST00000430233 ENST00000427374 ENST00000342665 ENST00000422694 ENST00000211402 ENST00000307340 ENST00000366943 ENST00000457796 ENST00000436128

Gene Information

This gene belongs to the RING finger family members of which encode proteins characterized by a RING domain a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1 EDR1 and CBX4 and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq Jul 2008]

Gene Symbol:   RING1
Gene Name:   ring finger protein 1
Aliases:   RING1A, RNF1
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000204227
Entrez:   6015
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.240000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download