PrimePCR™ SYBR® Green Assay: PFDN6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040009

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PFDN6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PFDN6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037286
Assay Design:   exonic
Chromosome Location:   6:33257566-33257691question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000445559 ENST00000452658 ENST00000448594 ENST00000491095 ENST00000547952 ENST00000399385 ENST00000399383 ENST00000383207 ENST00000482292 ENST00000548040 ENST00000395131 ENST00000374606 ENST00000374610 ENST00000463584 ENST00000374607 ENST00000412289 ENST00000425878 ENST00000442285 ENST00000474406 ENST00000552711 ENST00000431830 ENST00000432391 ENST00000451970 ENST00000468094 ENST00000547641

Gene Information

PFDN6 is a subunit of the heteromeric prefoldin complex that chaperones nascent actin (see MIM 102560) and alpha- and beta-tubulin (see MIM 602529 and MIM 191130 respectively) chains pending their transfer to the cytosolic chaperonin containing TCP1 (MIM 186980) (CCT) complex (Hansen et al. 1999 [PubMed 10209023]).[supplied by OMIM Jul 2010]

Gene Symbol:   PFDN6
Gene Name:   prefoldin subunit 6
Aliases:   H2-KE2, HKE2, KE-2, MGC70744, PFD6
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000204220
Entrez:   10471
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.710000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download