PrimePCR™ SYBR® Green Assay: GP1BB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040000

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037277
Assay Design:   exonic
Chromosome Location:   22:19712139-19712282question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000389840 ENST00000585328 ENST00000389665 ENST00000449205 ENST00000245046 ENST00000278947 ENST00000311481 ENST00000382549 ENST00000540881 ENST00000366425 ENST00000372073 ENST00000288381 ENST00000372068 ENST00000372072 ENST00000358598 ENST00000589228 ENST00000536854 ENST00000586397 ENST00000592800 ENST00000392711 ENST00000377564 ENST00000276974 ENST00000268893 ENST00000343736 ENST00000355701 ENST00000402364 ENST00000382292 ENST00000382298 ENST00000266744 ENST00000303941 ENST00000541565 ENST

Gene Information

Platelet glycoprotein Ib (GPIb) is a heterodimeric transmembrane protein consisting of a disulfide-linked 140 kD alpha chain and 22 kD beta chain. It is part of the GPIb-V-IX system that constitutes the receptor for von Willebrand factor (VWF) and mediates platelet adhesion in the arterial circulation. GPIb alpha chain provides the VWF binding site and GPIb beta contributes to surface expression of the receptor and participates in transmembrane signaling through phosphorylation of its intracellular domain. Mutations in the GPIb beta subunit have been associated with Bernard-Soulier syndrome velocardiofacial syndrome and giant platelet disorder. The 206 amino acid precursor of GPIb beta is synthesized from a 1.0 kb mRNA expressed in plateletes and megakaryocytes. A 411 amino acid protein arising from a longer unspliced transcript in endothelial cells has been described; however the authenticity of this product has been questioned. Yet another less abundant GPIb beta mRNA species of 3.5 kb expressed in nonhematopoietic tissues such as endothelium brain and heart was shown to result from inefficient usage of a non-consensus polyA signal in the neighboring upstream gene (SEPT5 septin 5). In the absence of polyadenylation from its own imperfect site the SEPT5 gene produces read-through transcripts that use the consensus polyA signal of this gene. [provided by RefSeq Dec 2010]

Gene Symbol:   GP1BB
Gene Name:   glycoprotein Ib (platelet), beta polypeptide
Aliases:   CD42C, GPIBB
RefSeq:   NC_000022.10 NG_007974.1 NT_011519.10
Ensembl:   ENSG00000203618
Entrez:   2812
UniGene:   Hs.283743
Chromosome Mapping:   22q11.21-q11.23,22q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.160000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download