PrimePCR™ SYBR® Green Assay: F8A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039991

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037268
Assay Design:   exonic
Chromosome Location:   X:154115738-154115868question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000317568 ENST00000355991 ENST00000416776 ENST00000531633 ENST00000369446 ENST00000369505 ENST00000369445 ENST00000370557 ENST00000318227 ENST00000448843 ENST00000380149 ENST00000597478 ENST00000598873 ENST00000599403

Gene Information

This gene is contained entirely within intron 22 of the factor VIII gene; spans less than 2 kb and is transcribed in the direction opposite of factor VIII. A portion of intron 22 (int22h) containing F8A is repeated twice extragenically closer to the Xq telomere. Although its function is unknown the observation that this gene is conserved in the mouse implies it has some function. Unlike factor VIII this gene is transcribed abundantly in a wide variety of cell types. [provided by RefSeq Jul 2008]

Gene Symbol:   F8A1
Gene Name:   coagulation factor VIII-associated 1
Aliases:   DXS522E, F8A, HAP40
RefSeq:   NC_000023.10 NG_005114.1 NG_011403.1 NT_167198.1
Ensembl:   ENSG00000197932
Entrez:   8263
UniGene:   Hs.533543
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.360000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download