PrimePCR™ SYBR® Green Assay: HIST1H4K, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039990

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037267
Assay Design:   exonic
Chromosome Location:   6:27799029-27799143question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000359907 ENST00000377364 ENST00000377803 ENST00000340756 ENST00000360441 ENST00000377745 ENST00000244537 ENST00000377727 ENST00000289352 ENST00000262442 ENST00000454412 ENST00000374161 ENST00000354348 ENST00000394329 ENST00000355057 ENST00000357549 ENST00000355981 ENST00000369165 ENST00000392939 ENST00000392938 ENST00000392933 ENST00000369157 ENST00000392932 ENST00000539745 ENST00000265678 ENST00000510118 ENST00000503859 ENST00000481261 ENST00000405189 ENST00000578186 ENST00000579512

Gene Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A H2B H3 and H4) form an octamer around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq Jul 2008]

Gene Symbol:   HIST1H4K
Gene Name:   histone cluster 1, H4k
RefSeq:   NC_000006.11 NG_000009.2 NT_007592.15
Ensembl:   ENSG00000197914
Entrez:   8362
UniGene:   Hs.528055
Chromosome Mapping:   6p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.670000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download