This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0039940
PrimePCR™ PreAmp for SYBR® Green Assay: F8A3, Human
PrimePCR™ Template for SYBR® Green Assay: F8A3, Human
This gene is part of a region that is repeated three times on chromosome X once in intron 22 of the F8 gene and twice closer to the Xq telomere. This record represents the most telomeric copy. Although its function is unknown the observation that this gene is conserved in the mouse implies it has some function. Unlike factor VIII this gene is transcribed abundantly in a wide variety of cell types. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.