PrimePCR™ SYBR® Green Assay: ARL17A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039935

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ARL17A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARL17A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037212
Assay Design:   exonic
Chromosome Location:   17:44653541-44653637question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000550273 ENST00000549665 ENST00000549904 ENST00000323456 ENST00000579925 ENST00000270590 ENST00000552373 ENST00000551700 ENST00000358894 ENST00000422202 ENST00000326996 ENST00000435874 ENST00000217289 ENST00000256413 ENST00000382998 ENST00000570618 ENST00000434041 ENST00000575698 ENST00000575960 ENST00000571246 ENST00000450673 ENST00000445552 ENST00000573185 ENST00000570550 ENST00000336125 ENST00000329240 ENST00000337845 ENST00000159111 ENST00000536461 ENST00000588361 ENST00000319977 ENST

Gene Information

Description Not Available

Gene Symbol:   ARL17A
Gene Name:   ADP-ribosylation factor-like 17A
Aliases:   ARF1P2, ARL17P1
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000185829
Entrez:   51326
Chromosome Mapping:   17q21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996200
y-intercept 37.090000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download