PrimePCR™ SYBR® Green Assay: ARL17A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039935

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037212
Assay Design:   exonic
Chromosome Location:   17:44653541-44653637question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000550273 ENST00000549665 ENST00000549904 ENST00000323456 ENST00000579925 ENST00000270590 ENST00000552373 ENST00000551700 ENST00000358894 ENST00000422202 ENST00000326996 ENST00000435874 ENST00000217289 ENST00000256413 ENST00000382998 ENST00000570618 ENST00000434041 ENST00000575698 ENST00000575960 ENST00000571246 ENST00000450673 ENST00000445552 ENST00000573185 ENST00000570550 ENST00000336125 ENST00000329240 ENST00000337845 ENST00000159111 ENST00000536461 ENST00000588361 ENST00000319977 ENST

Gene Information

Description Not Available

Gene Symbol:   ARL17A
Gene Name:   ADP-ribosylation factor-like 17A
Aliases:   ARF1P2, ARL17P1
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000185829
Entrez:   51326
UniGene:   Not Available
Chromosome Mapping:   17q21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996200
y-intercept 37.090000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download