PrimePCR™ SYBR® Green Assay: TCEB3C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039918

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TCEB3C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TCEB3C, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037195
Assay Design:   exonic
Chromosome Location:   18:44554957-44555106question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000591973 ENST00000451265 ENST00000330682 ENST00000602122 ENST00000361790 ENST00000360798 ENST00000347609 ENST00000427250 ENST00000354900 ENST00000334082 ENST00000424694 ENST00000404072 ENST00000445628

Gene Information

The SIII (or elongin) transcription elongation factor complex stimulates the rate of transcription elongation by RNA polymerase II by suppressing the transient pausing of the polymerase at many sites along the DNA template. This complex is a heterotrimer composed of the transcriptionally active subunit A A2 or A3 (or elongin A A2 or A3) and two regulatory subunits B and C (or elongin B and C). This gene encodes subunit A3. A3 and A are ubiquitously expressed whereas A2 is specifically expressed in the testis. [provided by RefSeq Mar 2010]

Gene Symbol:   TCEB3C
Gene Name:   transcription elongation factor B polypeptide 3C (elongin A3)
Aliases:   HsT829, TCEB3L2
RefSeq:   NC_000018.9 NG_016749.1 NT_010966.14
Ensembl:   ENSG00000183791
Entrez:   162699
Chromosome Mapping:   18q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.050000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download