PrimePCR™ SYBR® Green Assay: CTAG1A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039915

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037192
Assay Design:   exonic
Chromosome Location:   X:153814928-153815040question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000443287 ENST00000333128 ENST00000328435 ENST00000359887 ENST00000247306 ENST00000369585 ENST00000593606 ENST00000599837 ENST00000595485 ENST00000595847 ENST00000601408

Gene Information

CTAG1A is an identical copy of the CTAG1B gene (MIM 300156) (Aradhya et al. 2001 [PubMed 11709543]).[supplied by OMIM Mar 2008]

Gene Symbol:   CTAG1A
Gene Name:   cancer/testis antigen 1A
Aliases:   CTAG1B, ESO1, LAGE2A
RefSeq:   NC_000023.10 NT_167198.1
Ensembl:   ENSG00000183678
Entrez:   246100
UniGene:   Hs.534310
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.890000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download