PrimePCR™ SYBR® Green Assay: DEFB104B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039886

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DEFB104B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DEFB104B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037163
Assay Design:   exonic
Chromosome Location:   8:7327846-7327973question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000314265 ENST00000316169

Gene Information

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Defensins are short processed peptide molecules that are classified by structure into three groups: alpha-defensins beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. Chromosome 8p23 contains at least two copies of the duplicated beta-defensin cluster. This duplication results in two identical copies of defensin beta 104 DEFB104A and DEFB104B in head-to-head orientation. This gene DEFB104B represents the more telomeric copy. [provided by RefSeq Jul 2008]

Gene Symbol:   DEFB104B
Gene Name:   defensin, beta 104B
Aliases:   BD-4, DEFB-4, DEFB104A, hBD-4
RefSeq:   NC_000008.10 NG_005589.1 NT_023736.17
Ensembl:   ENSG00000177023
Entrez:   503618
Chromosome Mapping:   8p23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.980000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download