PrimePCR™ SYBR® Green Assay: AGAP8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039880

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AGAP8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AGAP8, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037157
Assay Design:   exonic
Chromosome Location:   10:51225438-51225558question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000456984 ENST00000453919 ENST00000304032 ENST00000336665 ENST00000409538 ENST00000418654 ENST00000453371 ENST00000428334 ENST00000602930 ENST00000425119 ENST00000448048 ENST00000374095 ENST00000412531 ENST00000374056 ENST00000452145 ENST00000413193 ENST00000355232 ENST00000443782 ENST00000374094 ENST00000342551 ENST00000311652 ENST00000584334 ENST00000581330

Gene Information

Description Not Available

Gene Symbol:   AGAP8
Gene Name:   ArfGAP with GTPase domain, ankyrin repeat and PH domain 8
Aliases:   CTGLF5
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000174194
Entrez:   728404
Chromosome Mapping:   10q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.170000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download