PrimePCR™ SYBR® Green Assay: RBMY1F, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039862

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RBMY1F, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RBMY1F, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037139
Assay Design:   exonic
Chromosome Location:   Y:24315146-24315679question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000381342 ENST00000383020 ENST00000382707 ENST00000303902 ENST00000439108 ENST00000382659 ENST00000382673 ENST00000382658 ENST00000382680 ENST00000382677 ENST00000418956 ENST00000303766 ENST00000454978 ENST00000250831 ENST00000414629 ENST00000445779

Gene Information

This gene encodes a protein containing an RNA-binding motif in the N-terminus and four SRGY (serine arginine glycine tyrosine) boxes in the C-terminus. Multiple copies of this gene are found in the AZFb azoospermia factor region of chromosome Y and the encoded protein is thought to be involved in spermatogenesis. Most copies of this locus are pseudogenes although six highly similar copies have full-length ORFs and are considered functional. Four functional copies of this gene are found within inverted repeat IR2; two functional copies of this gene are found in palindrome P3 along with two copies of PTPN13-like Y-linked. [provided by RefSeq Jul 2008]

Gene Symbol:   RBMY1F
Gene Name:   RNA binding motif protein, Y-linked, family 1, member F
Aliases:   MGC33094, YRRM2
RefSeq:   NC_000024.9 NG_004755.2 NT_011903.12
Ensembl:   ENSG00000169800
Entrez:   159163
Chromosome Mapping:   Yq11.223

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.960000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download