PrimePCR™ SYBR® Green Assay: CCDC103, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039850

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CCDC103, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CCDC103, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037127
Assay Design:   exonic
Chromosome Location:   17:42978364-42978892question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000306796 ENST00000354321 ENST00000538833 ENST00000464185 ENST00000402380 ENST00000157600 ENST00000415597 ENST00000454244 ENST00000535732 ENST00000397386 ENST00000349874 ENST00000397981 ENST00000397983 ENST00000397979 ENST00000545011 ENST00000577339 ENST00000410006 ENST00000357776 ENST00000410027 ENST00000417826 ENST00000331733 ENST00000304936 ENST00000366663 ENST00000470540 ENST00000427697 ENST00000409766

Gene Information

Description Not Available

Gene Symbol:   CCDC103
Gene Name:   coiled-coil domain containing 103
Aliases:   FLJ13094, FLJ34211
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000167131
Entrez:   388389
Chromosome Mapping:   17q21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.410000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download