PrimePCR™ SYBR® Green Assay: C2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039848

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037125
Assay Design:   exonic
Chromosome Location:   6:31901470-31901663question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000234256 ENST00000418584 ENST00000431247 ENST00000416252 ENST00000421932 ENST00000419062 ENST00000551081 ENST00000550363 ENST00000411885 ENST00000439426 ENST00000383362 ENST00000428349 ENST00000383367 ENST00000548973 ENST00000547984 ENST00000469372 ENST00000497706 ENST00000452323 ENST00000452202 ENST00000299367 ENST00000442278 ENST00000447952 ENST00000418949 ENST00000383177 ENST00000494905 ENST00000556679 ENST00000456570 ENST00000375510 ENST00000415985 ENST00000383359 ENST00000455713 ENST

Gene Information

Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.[provided by RefSeq Mar 2009]

Gene Symbol:   C2
Gene Name:   complement component 2
Aliases:   CO2, DKFZp779M0311
RefSeq:   NC_000006.11 NG_000013.3 NG_004658.2 NG_005163.2 NG_008191.1 NG_011730.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000166278
Entrez:   717
UniGene:   Hs.408903
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.030000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download