PrimePCR™ SYBR® Green Assay: TBC1D3G, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039841

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037118
Assay Design:   exonic
Chromosome Location:   17:34799052-34799555question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000374103 ENST00000419399 ENST00000574788 ENST00000250066 ENST00000332776 ENST00000304328 ENST00000454519 ENST00000398801 ENST00000336331 ENST00000457979 ENST00000308078 ENST00000451448 ENST00000394359 ENST00000455054 ENST00000535446 ENST00000400684 ENST00000394453 ENST00000592614 ENST00000341264 ENST00000330458 ENST00000535592 ENST00000535805 ENST00000327454 ENST00000505415 ENST00000378174 ENST00000539424 ENST00000518551 ENST00000354664 ENST00000519532 ENST00000537432 ENST00000339023 ENST

Gene Information

Description Not Available

Gene Symbol:   TBC1D3G
Gene Name:   TBC1 domain family, member 3G
Aliases:   Not Available
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000161583
Entrez:   654341
UniGene:   Hs.617010
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.830000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download