PrimePCR™ SYBR® Green Assay: CLEC18C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039837

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CLEC18C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CLEC18C, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037114
Assay Design:   exonic
Chromosome Location:   16:70208094-70208221question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000393701 ENST00000568461 ENST00000288040 ENST00000449317 ENST00000562923 ENST00000541793 ENST00000314151 ENST00000565806 ENST00000569347 ENST00000536907 ENST00000393853 ENST00000240335 ENST00000357260 ENST00000444059 ENST00000261413 ENST00000457646 ENST00000339953 ENST00000310164 ENST00000233623 ENST00000410003 ENST00000296387

Gene Information

Description Not Available

Gene Symbol:   CLEC18C
Gene Name:   C-type lectin domain family 18, member C
Aliases:   MGC34761, MRCL, MRCL3
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000157335
Entrez:   283971
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.700000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download