PrimePCR™ SYBR® Green Assay: XAGE2B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039836

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037113
Assay Design:   exonic
Chromosome Location:   X:52112185-52112299question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000491143 ENST00000262095 ENST00000289292 ENST00000460112 ENST00000376020 ENST00000286049 ENST00000330906 ENST00000373468 ENST00000055682 ENST00000277225 ENST00000374686 ENST00000441147 ENST00000457913 ENST00000542028 ENST00000601085 ENST00000600171 ENST00000596464 ENST00000596650

Gene Information

Description Not Available

Gene Symbol:   XAGE2B
Gene Name:   X antigen family, member 2B
Aliases:   XAGE2
RefSeq:   NC_000023.10 NT_011638.13
Ensembl:   ENSG00000155622
Entrez:   728242
UniGene:   Hs.522654
Chromosome Mapping:   Xp11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.690000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download