PrimePCR™ SYBR® Green Assay: MAGED4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039834

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MAGED4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MAGED4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037111
Assay Design:   exonic
Chromosome Location:   X:51929768-51929867question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000242839 ENST00000400366 ENST00000344297 ENST00000497164 ENST00000360134 ENST00000485287 ENST00000335504 ENST00000486010 ENST00000431659 ENST00000375626 ENST00000416960 ENST00000406846 ENST00000346173 ENST00000263713 ENST00000452780 ENST00000601264 ENST00000595965 ENST00000601229 ENST00000593675 ENST00000600173 ENST00000594654 ENST00000599522

Gene Information

Description Not Available

Gene Symbol:   MAGED4
Gene Name:   melanoma antigen family D, 4
Aliases:   FLJ39060, KIAA1859, MAGE-E1, MAGE1, MAGEE1, MGC3210
RefSeq:   NC_000023.10 NT_011638.13
Ensembl:   ENSG00000154545
Entrez:   728239
Chromosome Mapping:   Xp11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.970000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download