PrimePCR™ SYBR® Green Assay: RGPD3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039830

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037107
Assay Design:   exonic
Chromosome Location:   2:107084705-107084795question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000304514 ENST00000409886 ENST00000354986 ENST00000408999 ENST00000568259 ENST00000283195 ENST00000393283 ENST00000430736 ENST00000016946 ENST00000441344 ENST00000272454 ENST00000329516 ENST00000330331 ENST00000446930 ENST00000217446 ENST00000374820 ENST00000302558 ENST00000330575 ENST00000496537 ENST00000546530 ENST00000261729 ENST00000446861 ENST00000548055 ENST00000243077 ENST00000397708 ENST00000291688 ENST00000379540 ENST00000379521 ENST00000318524 ENST00000394967 ENST00000595354 ENST

Gene Information

This gene is located in a cluster of Ran-binding protein related genes on chromosome 2 which arose through duplication in primates. The encoded protein contains an N-terminal TPR (tetratricopeptide repeat) domain two Ran-binding domains and a C-terminal GRIP domain (golgin-97 RanBP2alpha Imh1p and p230/golgin-245) domain. [provided by RefSeq Sep 2011]

Gene Symbol:   RGPD3
Gene Name:   RANBP2-like and GRIP domain containing 3
Aliases:   RGP3
RefSeq:   NC_000002.11 NG_021160.1 NT_022171.15
Ensembl:   ENSG00000153165
Entrez:   653489
UniGene:   Hs.726367
Chromosome Mapping:   2q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.640000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download