PrimePCR™ SYBR® Green Assay: FLOT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039815

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FLOT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FLOT1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037092
Assay Design:   exonic
Chromosome Location:   6:30708280-30708461question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000430843 ENST00000457733 ENST00000426417 ENST00000431966 ENST00000430384 ENST00000411848 ENST00000444068 ENST00000376389 ENST00000413165 ENST00000438162 ENST00000418160 ENST00000445853 ENST00000416018 ENST00000454845 ENST00000456573 ENST00000446072 ENST00000457133 ENST00000432428 ENST00000437093 ENST00000422714 ENST00000436025 ENST00000553223 ENST00000383382 ENST00000422636 ENST00000454249 ENST00000438134 ENST00000421565 ENST00000416389 ENST00000439743 ENST00000444632 ENST00000444497 ENST

Gene Information

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   FLOT1
Gene Name:   flotillin 1
Aliases:   Not Available
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000137312
Entrez:   10211
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.460000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download