PrimePCR™ SYBR® Green Assay: ZNRD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039781

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNRD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNRD1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037058
Assay Design:   exonic
Chromosome Location:   6:30029305-30029444question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000437373 ENST00000431032 ENST00000433264 ENST00000432545 ENST00000432904 ENST00000420100 ENST00000432227 ENST00000444027 ENST00000443494 ENST00000437507 ENST00000443142 ENST00000453694 ENST00000332435 ENST00000376782 ENST00000359374 ENST00000376785 ENST00000455948 ENST00000428913 ENST00000446493 ENST00000451875 ENST00000400662 ENST00000383613 ENST00000400660 ENST00000400659 ENST00000417738 ENST00000429558 ENST00000441251 ENST00000431416 ENST00000417275 ENST00000444794 ENST00000442585 ENST

Gene Information

This gene encodes a protein with similarity to the Saccharomyces cerevisiae Rpa12p subunit of RNA polymerase I. Alternate splicing of this gene results in two transcript variants encoding the same protein. Additional splice variants have been described but their full-length sequences have not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   ZNRD1
Gene Name:   zinc ribbon domain containing 1
Aliases:   HTEX-6, MGC13376, Rpa12, TEX6, hZR14, tctex-6
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000066379
Entrez:   30834
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.000000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download