PrimePCR™ SYBR® Green Assay: RP11-1109F11.4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039771

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RP11-1109F11.4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RP11-1109F11.4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037048
Assay Design:   exonic
Chromosome Location:   12:89919869-89919975question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000548729 ENST00000547474 ENST00000413530

Gene Information

This locus represents naturally occurring transcripts that splice the 5' exons of the POC1B (POC1 centriolar protein homolog B) gene on chromosome 12 to the GALNT4 (UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4) gene which is located within a POC1B intron. Alternative splicing results in two transcript variants one of which encodes a fusion isoform that shares sequence identity with the products of each individual gene. [provided by RefSeq Dec 2010]

Gene Symbol:   RP11-1109F11.4
Gene Name:   POC1B-GALNT4 protein isoform 1
Aliases:   DKFZp686D0362, FLJ58458
RefSeq:   Not Available
Ensembl:   ENSG00000259075
Entrez:   100528030
Chromosome Mapping:   12q

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.750000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download