PrimePCR™ SYBR® Green Assay: HAUS5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039746

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HAUS5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HAUS5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037022
Assay Design:   exonic
Chromosome Location:   19:36114219-36114323question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000203166 ENST00000590994 ENST00000379045

Gene Information

HAUS5 is 1 of 8 subunits of the 390-kD human augmin complex or HAUS complex. The augmin complex was first identified in Drosophila and its name comes from the Latin verb 'augmentare' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al. 2008 [PubMed 18443220]; Uehara et al. 2009 [PubMed 19369198]).[supplied by OMIM Jun 2010]

Gene Symbol:   HAUS5
Gene Name:   HAUS augmin-like complex, subunit 5
Aliases:   KIAA0841, dgt5
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000249115
Entrez:   23354
Chromosome Mapping:   19q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.980000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download