This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0039746
PrimePCR™ PreAmp for SYBR® Green Assay: HAUS5, Human
PrimePCR™ Template for SYBR® Green Assay: HAUS5, Human
HAUS5 is 1 of 8 subunits of the 390-kD human augmin complex or HAUS complex. The augmin complex was first identified in Drosophila and its name comes from the Latin verb 'augmentare' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al. 2008 [PubMed 18443220]; Uehara et al. 2009 [PubMed 19369198]).[supplied by OMIM Jun 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.