This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0039675
PrimePCR™ PreAmp for SYBR® Green Assay: SLC9A8, Human
PrimePCR™ Template for SYBR® Green Assay: SLC9A8, Human
Sodium-hydrogen exchangers (NHEs) such as SLC9A8 are integral transmembrane proteins that exchange extracellular Na+ for intracellular H+. NHEs have multiple functions including intracellular pH homeostasis cell volume regulation and electroneutral NaCl absorption in epithelia (Xu et al. 2008 [PubMed 18209477]).[supplied by OMIM Apr 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.