This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0039662
PrimePCR™ PreAmp for SYBR® Green Assay: ADH1B, Human
PrimePCR™ Template for SYBR® Green Assay: ADH1B, Human
The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates including ethanol retinol other aliphatic alcohols hydroxysteroids and lipid peroxidation products. This encoded protein consisting of several homo- and heterodimers of alpha beta and gamma subunits exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.