PrimePCR™ SYBR® Green Assay: NAT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039513

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NAT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NAT1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036789
Assay Design:   exonic
Chromosome Location:   8:18079565-18079710question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000307719 ENST00000518029 ENST00000517492 ENST00000520546 ENST00000545197 ENST00000535084 ENST00000539092 ENST00000541942

Gene Information

This gene is one of two arylamine N-acetyltransferase (NAT) genes in the human genome and is orthologous to the mouse and rat Nat2 genes. The enzyme encoded by this gene catalyzes the transfer of an acetyl group from acetyl-CoA to various arylamine and hydrazine substrates. This enzyme helps metabolize drugs and other xenobiotics and functions in folate catabolism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   NAT1
Gene Name:   N-acetyltransferase 1 (arylamine N-acetyltransferase)
Aliases:   AAC1, MNAT, NAT-1, NATI
RefSeq:   NC_000008.10 NT_167187.1 NG_012245.1
Ensembl:   ENSG00000171428
Entrez:   9
Chromosome Mapping:   8p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.560000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download