This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0039509
PrimePCR™ PreAmp for SYBR® Green Assay: HS6ST2, Human
PrimePCR™ Template for SYBR® Green Assay: HS6ST2, Human
Heparan sulfate proteoglycans are ubiquitous components of the cell surface extracellular matrix and basement membranes and interact with various ligands to influence cell growth differentiation adhesion and migration. This gene encodes a member of the heparan sulfate (HS) sulfotransferase gene family which catalyze the transfer of sulfate to HS. Different family members and isoforms are thought to synthesize heparan sulfates with tissue-specific structures and functions. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.