PrimePCR™ SYBR® Green Assay: EFNA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039491

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036767
Assay Design:   exonic
Chromosome Location:   1:155106753-155106847question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000368407 ENST00000368406

Gene Information

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships ephrins are divided into the ephrin-A (EFNA) class which are anchored to the membrane by a glycosylphosphatidylinositol linkage and the ephrin-B (EFNB) class which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2 EPHA4 EPHA5 EPHA6 and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis. [provided by RefSeq Jul 2008]

Gene Symbol:   EFNA1
Gene Name:   ephrin-A1
Aliases:   B61, ECKLG, EFL1, EPLG1, LERK1, TNFAIP4
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000169242
Entrez:   1942
UniGene:   Hs.516664
Chromosome Mapping:   1q21-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.980000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download