PrimePCR™ SYBR® Green Assay: UGT2B4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039392

List Price:    $174.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036668
Assay Design:   exonic
Chromosome Location:   4:70346275-70346396question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000512583 ENST00000305107 ENST00000381096

Gene Information

Description Not Available

Gene Symbol:   UGT2B4
Gene Name:   UDP glucuronosyltransferase 2 family, polypeptide B4
Aliases:   HLUG25, UDPGT2B4, UDPGTH1, UGT2B11
RefSeq:   NC_000004.11 NT_022778.16
Ensembl:   ENSG00000156096
Entrez:   7363
Chromosome Mapping:   4q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.650000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download