This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0039349
PrimePCR™ PreAmp for SYBR® Green Assay: ASB6, Human
PrimePCR™ Template for SYBR® Green Assay: ASB6, Human
The protein encoded by this gene belongs to a family of ankyrin repeat proteins that along with four other protein families contain a C-terminal SOCS box motif. Growing evidence suggests that the SOCS box similar to the F-box acts as a bridge between specific substrate-binding domains and the more generic proteins that comprise a large family of E3 ubiquitin protein ligases. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq Jan 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.