PrimePCR™ SYBR® Green Assay: TBC1D7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039337

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036613
Assay Design:   exonic
Chromosome Location:   6:13321199-13321293question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000356436 ENST00000379300 ENST00000452989 ENST00000450347 ENST00000422136 ENST00000446018 ENST00000420456 ENST00000428109 ENST00000416436 ENST00000379291 ENST00000421203 ENST00000379307 ENST00000343141

Gene Information

TBC1D7 belongs to a family of proteins sharing a 180- to 200-amino acid TBC domain presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2 (USP6; MIM 604334) yeast Bub2 and CDC16 (MIM 603461) (Nakashima et al. 2007 [PubMed 17658474]).[supplied by OMIM Jul 2010]

Gene Symbol:   TBC1D7
Gene Name:   TBC1 domain family, member 7
Aliases:   DKFZp686N2317, FLJ32666, PIG51, TBC7, dJ257A7.3
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000145979
Entrez:   51256
UniGene:   Hs.484678
Chromosome Mapping:   6p24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.670000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download