PrimePCR™ SYBR® Green Assay: CSF3R, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039185

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036461
Assay Design:   exonic
Chromosome Location:   1:36933677-36933818question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000373106 ENST00000373104 ENST00000373103 ENST00000464465 ENST00000331941 ENST00000361632 ENST00000418048 ENST00000440588 ENST00000338937

Gene Information

The protein encoded by this gene is the receptor for colony stimulating factor 3 a cytokine that controls the production differentiation and function of granulocytes. The encoded protein which is a member of the family of cytokine receptors may also function in some cell surface adhesion or recognition processes. Alternatively spliced transcript variants have been described. Mutations in this gene are a cause of Kostmann syndrome also known as severe congenital neutropenia. [provided by RefSeq Aug 2010]

Gene Symbol:   CSF3R
Gene Name:   colony stimulating factor 3 receptor (granulocyte)
Aliases:   CD114, GCSFR
RefSeq:   NC_000001.10 NT_032977.9 NG_016270.1
Ensembl:   ENSG00000119535
Entrez:   1441
UniGene:   Hs.524517
Chromosome Mapping:   1p35-p34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.690000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download