PrimePCR™ SYBR® Green Assay: LHX6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039124

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LHX6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LHX6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036400
Assay Design:   exonic
Chromosome Location:   9:124966584-124966724question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000373755 ENST00000373754 ENST00000340587 ENST00000394319 ENST00000541397 ENST00000464484 ENST00000482062

Gene Information

This gene encodes a member of a large protein family that contains the LIM domain a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and may be involved in the control of differentiation and development of neural and lymphoid cells. Two alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. Alternatively spliced transcript variants have been identified but their biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   LHX6
Gene Name:   LIM homeobox 6
Aliases:   LHX6.1, MGC119542, MGC119544, MGC119545
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000106852
Entrez:   26468
Chromosome Mapping:   9q33.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.680000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download