PrimePCR™ SYBR® Green Assay: SNX11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039008

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036284
Assay Design:   exonic
Chromosome Location:   17:46196404-46196501question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000581298 ENST00000580219 ENST00000452859 ENST00000393405 ENST00000359238 ENST00000582104 ENST00000584335 ENST00000582481 ENST00000439357

Gene Information

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain which is a phosphoinositide binding domain and are involved in intracellular trafficking. This protein does not contain a coiled coil region like some family members. This gene encodes a protein of unknown function. This gene results in two transcript variants differing in the 5' UTR but encoding the same protein. [provided by RefSeq Jul 2008]

Gene Symbol:   SNX11
Gene Name:   sorting nexin 11
Aliases:   MGC111019
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000002919
Entrez:   29916
UniGene:   Hs.15827
Chromosome Mapping:   17q21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.980000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download