PrimePCR™ SYBR® Green Assay: ACTB, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0036280

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Assay Information

Technology:   qPCR
Assay Type   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036269
Assay Design:   Exonic
Chromosome Location:   7:5568936-5569027question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000290378 ENST00000331789 ENST00000432588 ENST00000443528 ENST00000417101 ENST00000414620

Gene Information

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility structure and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq Jul 2008]

Gene Symbol:   ACTB
Gene Name:   actin, beta
Aliases:   PS1TP5BP1
RefSeq:   NC_000007.13 NG_007992.1 NT_007819.17
Ensembl:   ENSG00000075624
Entrez:   60
UniGene:   Hs.520640
Chromosome Mapping   7p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.993900
y-intercept 36.140000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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