This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: MAGEE1, Human
PrimePCR™ Template for SYBR® Green Assay: MAGEE1, Human
This gene encodes an alpha-dystrobrevin-associated MAGE (melanoma-associated antigen) protein which is a member of the MAGE family. The protein contains a nuclear localization signal in the N-terminus 30 12-amino acid repeats beginning at nt 60 with the consensus sequence ASEGPSTSVLPT and two MAGE domains in the C-terminus. It may play a signaling role in brain muscle and peripheral nerve. This gene is located on X chromosome in a region containing loci linked to mental retardation. [provided by RefSeq Mar 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.