PrimePCR™ SYBR® Green Assay: MAGEE1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0034389
Assay Design:   Exonic
Chromosome Location:   X:75650495-75650593question
Amplicon Length:   69

Gene Information

This gene encodes an alpha-dystrobrevin-associated MAGE (melanoma-associated antigen) protein which is a member of the MAGE family. The protein contains a nuclear localization signal in the N-terminus 30 12-amino acid repeats beginning at nt 60 with the consensus sequence ASEGPSTSVLPT and two MAGE domains in the C-terminus. It may play a signaling role in brain muscle and peripheral nerve. This gene is located on X chromosome in a region containing loci linked to mental retardation. [provided by RefSeq Mar 2010]

Gene Symbol:   MAGEE1
Gene Name:   melanoma antigen family E, 1
Aliases:   DAMAGE, HCA1, KIAA1587
RefSeq:   NC_000023.10 NG_016774.1 NT_011669.17
Ensembl:   ENSG00000198934
Entrez:   57692
UniGene:   Hs.8453
Chromosome Mapping:   Xq13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.830000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download