This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: BEST3, Human
PrimePCR™ Template for SYBR® Green Assay: BEST3, Human
BEST3 belongs to the bestrophin family of anion channels which includes BEST1 (MIM 607854) the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700) and 2 other BEST1-like genes BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al. 2002 [PubMed 12032738]; Tsunenari et al. 2003 [PubMed 12907679]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.