PrimePCR™ SYBR® Green Assay: BEST3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0034012
Assay Design:   Exonic
Chromosome Location:   12:70072544-70072642question
Amplicon Length:   69

Gene Information

BEST3 belongs to the bestrophin family of anion channels which includes BEST1 (MIM 607854) the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700) and 2 other BEST1-like genes BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al. 2002 [PubMed 12032738]; Tsunenari et al. 2003 [PubMed 12907679]).[supplied by OMIM Mar 2008]

Gene Symbol:   BEST3
Gene Name:   bestrophin 3
Aliases:   MGC13168, MGC40411, VMD2L3
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000127325
Entrez:   144453
UniGene:   Hs.280782
Chromosome Mapping:   12q14.2-q15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.060000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download